In addition, we demonstrated that the RNAi effect can also be achieved by generating RNAi-active miRNA mimics, which are chemically synthetic miRNAs able to induce RNAi phenomena. Here, an artificial miRNA construct mimicking miR-21 was used to inhibit AMO-induced RNAi. Interestingly, the mimic was found to interfere with the effect of the AMO on RNAi induction, suggesting that AMO and synthetic mimics (AMOs) act by a different mechanism. Finally, we demonstrated that synthetic AMOs are able to inhibit miR-21 expression in vivo. Thus, synthetic AMOs can be used to inhibit miRNA function in vivo.
By regulating the activity of miRNAs, siRNAs or AMOs can be used to control the expression of specific genes to regulate disease pathogenesis or progression. In contrast, a miRNA or siRNA that is no longer complementary to the target mRNA results in the degradation of the targeted mRNA and thus removes miRNAs from the target mRNA complex and reduces the efficiency of its translation.
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You can find the children's short story collection for free. These stories are a great reference material for students who need to prepare for their major examinations and for children who are preparing for the National Kindergarten Examinations.
As we all know, eBooks are wonderful tools in our technology assisted schoolwork. As students, we need to read more, and in order to do that, we need to develop some strategies to get our hands on those books. In this section, we have a collection of different eBook resources that can be used by students.
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